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PD Dr. Isaac Bugueno Valdebenito, PhD

Senior Teaching and Research Assistant

PD Dr. Isaac Bugueno Valdebenito, PhD
Contact: E-Mail: isaac.buguenovaldebenito@zzm.uzh.ch
Qualifications: PD, PhD, MSc, DDS 
Affiliation: Institute of Oral Biology / 
Orofacial Development and Regeneration

Clinical Specialisations

Periodontology & Oral Implantology; Dental care for genetic rare disorders

Research Interests

  • Oro-dental rare genetic diseases
  • Orofacial tissue regeneration
  • Functional biomaterials
  • Stem cell biology
  • Advanced in vitro 3D models and microfluidics
  • Oral physiopathology

Orofacial development and tissue regeneration, with a focus on the cellular and molecular mechanisms governing tooth and craniofacial development. Stem cells-based approaches for dental and craniofacial regeneration, including dental pulp and mesenchymal stromal cells, and the study of epithelio-mesenchymal crosstalk during odontogenesis in physiological and pathological contexts. Oro-dental rare genetic diseases, particularly enamel and dentin defects, and their modeling to elucidate diseases mechanisms. We use advanced in vitro organotypic and microfluidic models, to bridge developmental biology and translational regenerative dentistry.

Selected publications

Nikolic S, Alastra G, Pultar F, Lüthy L, Stadlinger B, Carreira EM, Bugueno IM*, Mitsiadis TA* (2025). Mutanobactin-D, a Streptococcus mutans Non-Ribosomal Cyclic Lipopeptide, Induces Osteogenic/Odontogenic Differentiation of Human Dental Pulp Stem Cells and Human Bone Marrow Stem Cells. Int. J. Mol. Sci. 2025, 26(3), 1144. DOI: 10.3390/ijms26031144. PMID: 39940912.

Bugueno IM, Rey T, Jimenez-Armijo A, Kawczynski M, Kharouf N, Manière MC, Hérault Y, Bloch-Zupan A, Haushalter-Laugel V (2024). Rare dentin defects: understanding the pathophysiological mechanisms of COLXVA1 mutations. Genes & Diseases 2024 Sep; 11(5): 101303. doi: 10.1016/j.gendis.2024.101303. PMID: 38721411.

Bugueno IM, Alastra G, Balic A, Stadlinger B, Mitsiadis TA (2024). Limited adipogenic differentiation potential of human dental pulp stem cells compared to human bone marrow stem cells. Int J Mol Sci. 2024 Oct 16;25(20):11105. DOI: 10.3390/ijms252011105. PMID: 39456888.

Jerbaka F, Grivoba V, Rey T, El-Faloussi S, Herault Y, Bloch-Zupan A, Bugueno IM (2024). Organotypic 3D Cellular Models Mimicking the Epithelio-Ectomesenchymal Bilayer During Odontogenesis. Tissue Eng Part A. 2024 9 30. DOI: 10.1089/ten.TEA.2024.0118. PMID: 39276088.

Bloch-Zupan A, Rey T, Jimenez-Armijo A, Kawczynski M, Kharouf N, O-Rare consortium, ERN Cranio consortium: Dostalova T, Macek Jr M; International consortium: El Alloussi M, Qebibo L, Morkmued S, Pungchanchaikul P, Revencu N, Urzúa Orellana B, Gérard B, Manière MC., Bugueno IM, Laugel-Haushalter V (2023). Amelogenesis imperfecta: Next-generation sequencing sheds light on Witkop’s classification. Front Physiol. 2023 May 9:14:1130175. DOI: 10.3389/fphys.2023.1130175. PMID: 37228816.

Bugueno IM, El-Ghazouani F, Batool F, El Itawi H, Anglès-Cano E, Benkirane-Jessel N, Toti F, Huck O (2020). Porphyromonas gingivalis triggers the shedding of inflammatory endothelial microvesicles that act as autocrine effectors of endothelial dysfunction. Scientific Reports 2020 4;10(1):1778. DOI: 10.1038/s41598-020-58374-z. PMID: 32019950.

Publications in ZORA (Zurich Open Repository and Archive)

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